7BY3

Toxin-antitoxin complex from klebsiella pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	193
Detector technology	CCD
Collection date	2017-09-28
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	110.435, 79.019, 59.609
Unit cell angles	90.00, 89.98, 90.00

Refinement procedure

Resolution	50.000 - 2.000
Rwork	0.244
R-free	0.27923
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.220
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.080
High resolution limit [Å]	2.000	2.040
Rpim	0.037	0.267
Number of reflections	31667	1644
<I/σ(I)>	48.3
Completeness [%]	96.8
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Tris