7BY1

Crystal structure of GCN5 PCAF N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-18
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.1000
Spacegroup name	P 1 21 1
Unit cell lengths	43.069, 148.857, 58.998
Unit cell angles	90.00, 101.03, 90.00

Refinement procedure

Resolution	45.700 - 1.800
R-factor	0.1978
Rwork	0.196
R-free	0.23148
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.777
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHARP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.700	1.840
High resolution limit [Å]	1.800	1.800
Number of reflections	67271	28263
<I/σ(I)>	14.7
Completeness [%]	100.0
Redundancy	7
CC(1/2)	0.998	0.794

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM imidazole/MES (pH 6.5), 20% PEGMME500, 10% PEG20,000 , 20 mM sodium formate , 20 mM ammonium acetate, 20 mM sodium citrate tribasic dehydrate, 20 mM sodium potassium tartrate tetrahydrate, 20 mM sodium oxamate