7BWJ

crystal structure of SARS-CoV-2 antibody with RBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9796
Spacegroup name	P 21 21 21
Unit cell lengths	70.230, 90.150, 112.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.552 - 2.850
R-factor	0.2214
Rwork	0.219
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m0j
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.150	2.920
High resolution limit [Å]	2.850	2.850
Rmerge	0.154	1.452
Number of reflections	17230	1273
<I/σ(I)>	14.3
Completeness [%]	99.9
Redundancy	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2M magnesium formate dihydrate, 0.1M sodium acetate trihydrate, pH 4.0, 18% PEG 5000 mme