7BWG
A Glycoside Hydrolase Family 20 beta-N-Acetylglucosaminidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-03-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.035, 81.928, 79.418 |
| Unit cell angles | 90.00, 94.08, 90.00 |
Refinement procedure
| Resolution | 57.610 - 2.850 |
| R-factor | 0.2942 |
| Rwork | 0.292 |
| R-free | 0.33450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hp4 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.642 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.610 | 2.950 |
| High resolution limit [Å] | 2.848 | 2.848 |
| Rmerge | 0.229 | 0.773 |
| Rmeas | 0.252 | 0.845 |
| Number of reflections | 23477 | 2334 |
| <I/σ(I)> | 8.19 | 2.62 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.1 | |
| CC(1/2) | 0.982 | 0.864 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M MES pH 6.5, 10% (V/V) Dioxane, 1.6 M (NH4)2SO4 |
