7BWF

YoeB-YefM complex from Staphylococcus aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-31
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97942
Spacegroup name	P 31
Unit cell lengths	67.849, 67.849, 69.356
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.832 - 1.700
R-factor	0.207
Rwork	0.206
R-free	0.22960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a6q
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.069	0.611
Number of reflections	38302	1944
<I/σ(I)>	26.9	2.3
Completeness [%]	97.4	99
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.08 M sodium chloride, 0.02 M magnesium chloride hexahydrate, 0.04 M sodium cacodylate trihydrate pH 7.0, 40% v/v (+/-)-2-methyl-2,4-pentanediol, and 0.012 M spermine tetrahydrochloride