7BWB
Bombyx mori GH32 beta-fructofuranosidase BmSUC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-06 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 94.752, 119.308, 100.275 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.872 - 1.800 |
| Rwork | 0.168 |
| R-free | 0.21310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uyp |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.523 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.051 | 0.607 |
| Number of reflections | 52834 | 7635 |
| <I/σ(I)> | 17 | 2.5 |
| Completeness [%] | 99.9 | |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | 13% PEG 3350, 40 mM citric acid, 60 mM Bis-Tris propane |
