7BVL

Crystal structure of glucose isomerase delivered in wheat starch

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	298.15
Detector technology	PIXEL
Collection date	2019-05-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	I 2 2 2
Unit cell lengths	94.490, 99.920, 100.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.749 - 2.000
R-factor	0.1731
Rwork	0.171
R-free	0.21300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zyd
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.400	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	33248	3247
<I/σ(I)>	4.01
Completeness [%]	100.0
Redundancy	148.5
CC(1/2)	0.976	0.597

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7	293.5	Tris-HCl, Ammonium sulfate, Magnesium sulfate