7BVD
Anthranilate synthase component I (TrpE)[Mycolicibacterium smegmatis]
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.822, 66.076, 80.718 |
| Unit cell angles | 81.88, 72.73, 70.88 |
Refinement procedure
| Resolution | 38.490 - 1.700 |
| Rwork | 0.164 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cwa |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.034 |
| Data reduction software | HKL-3000 (v720) |
| Data scaling software | HKL-3000 (v720) |
| Phasing software | PHENIX (version 1.17.1-3660) |
| Refinement software | PHENIX (version 1.17.1-3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.063 | 0.388 |
| Rpim | 0.040 | 0.244 |
| Number of reflections | 121727 | 5900 |
| <I/σ(I)> | 19.4 | 2.1 |
| Completeness [%] | 97.3 | 94.4 |
| Redundancy | 3.5 | 3.3 |
| CC(1/2) | 0.990 | 0.910 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Magnesium formate dihydrate, 15% w/v Polyethylene glycol 3350 |
