7BV6

Crystal structure of the autophagic STX17/SNAP29/VAMP8 SNARE complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97776
Spacegroup name	P 21 21 2
Unit cell lengths	143.981, 196.000, 76.142
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.000 - 3.050
R-factor	0.1792
Rwork	0.175
R-free	0.25540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gl2
RMSD bond length	0.009
RMSD bond angle	0.999
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.100
High resolution limit [Å]	3.050	3.050
Number of reflections	41797
<I/σ(I)>	6.3
Completeness [%]	99.9
Redundancy	6.6
CC(1/2)	0.990	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	2.5 M Ammonium nitrate, 0.1 M Sodium acetate trihydrate pH 4.6