7BUW
Eucommia ulmoides TPT3 mutant -C94Y/A95F
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-01-30 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 84.253, 84.253, 181.189 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.530 - 3.300 |
R-factor | 0.2556 |
Rwork | 0.253 |
R-free | 0.31040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4kk2 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.777 |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.530 | 3.418 |
High resolution limit [Å] | 3.300 | 3.300 |
Number of reflections | 11760 | 1137 |
<I/σ(I)> | 12.2 | |
Completeness [%] | 99.9 | |
Redundancy | 21.1 | |
CC(1/2) | 0.996 | 0.783 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | CHES, PEG 8000, Risedronate, MgCl2 |