7BUP

Crystal structure of PCNA from pathogenic yeast Candida albicans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	103
Detector technology	CCD
Collection date	2017-05-18
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	78.520, 78.910, 150.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.170 - 2.700
R-factor	0.2215
Rwork	0.219
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yhr
Data reduction software	iMOSFLM (7.2.1)
Data scaling software	Aimless (0.5.29)
Phasing software	MOLREP (11.5.06)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.170	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.031	0.244
Rmeas	0.044	0.345
Rpim	0.031	0.244
Number of reflections	26417	2611
<I/σ(I)>	2.8	2.8
Completeness [%]	100.0	99.96
Redundancy	2	2
CC(1/2)	0.999	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	20 % V/V Glycerol 24 % PEG 1500