7BUH
Reduced ferredoxin of carbazole 1,9a-dioxygenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2016-07-20 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 61.045, 67.725, 45.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.340 - 1.790 |
| R-factor | 0.1594 |
| Rwork | 0.157 |
| R-free | 0.20110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vck |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.825 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.450 | 1.830 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.150 | 0.829 |
| Number of reflections | 9143 | 532 |
| <I/σ(I)> | 29.6 | |
| Completeness [%] | 99.9 | |
| Redundancy | 26.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Buffer: 5 mM Tris pH 7.5, Reservior: 0.2 M Ammonium acetate, 0.1 M Sodium acetate trihydrate pH 4.1, 30% w/v Polyethylene glycol 4000 |
