7BU9

Crystal Structure of Spindlin1-H3(K4me3-K9me2) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2019-02-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	40.471, 143.783, 129.990
Unit cell angles	90.00, 95.86, 90.00

Refinement procedure

Resolution	48.074 - 3.502
R-factor	0.2466
Rwork	0.246
R-free	0.26420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ns2
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3247)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.560
High resolution limit [Å]	3.500	9.480	3.500
Rmerge	0.153	0.068	0.490
Rmeas	0.181	0.081	0.578
Rpim	0.096	0.043	0.304
Total number of observations	64751
Number of reflections	18492	968	930
<I/σ(I)>	4.5
Completeness [%]	98.9	99.4	99.9
Redundancy	3.5	3.4	3.6
CC(1/2)		0.996	0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	291	3.6M sodium formate, 3% DMSO