7BU6
Structure of human beta1 adrenergic receptor bound to norepinephrine and nanobody 6B9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-12-12 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 376.779, 66.009, 47.841 |
| Unit cell angles | 90.00, 93.46, 90.00 |
Refinement procedure
| Resolution | 19.980 - 2.700 |
| R-factor | 0.2334 |
| Rwork | 0.232 |
| R-free | 0.25840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lde |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.838 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 32544 | 3316 |
| <I/σ(I)> | 10.32 | 0.99 |
| Completeness [%] | 100.0 | |
| Redundancy | 41.6 | |
| CC(1/2) | 0.995 | 0.518 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 100 mM HEPES, pH 7.5, 100-250 mM Sodium Sulfate, 39-43% PEG300, 10mM norepinephrine |
