7BTC
Crystal structure of Rheb Y35N mutant bound to GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.426, 78.656, 117.398 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.328 - 2.101 |
| R-factor | 0.1888 |
| Rwork | 0.187 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xtq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.982 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
| Rmerge | 0.108 | 0.066 | 0.572 |
| Rmeas | 0.113 | 0.069 | 0.602 |
| Rpim | 0.031 | 0.020 | 0.182 |
| Total number of observations | 524442 | ||
| Number of reflections | 41416 | 2269 | 1998 |
| <I/σ(I)> | 4.1 | ||
| Completeness [%] | 99.9 | 100 | 97.7 |
| Redundancy | 12.7 | 12.2 | 9.7 |
| CC(1/2) | 0.999 | 0.902 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.5 | 290 | 0.10% n-Octyl-beta-D-glucoside: 0.1 M Sodium citrate tribasic, pH 5.5: 22% PEG 3350 |
