7BSO

Crystal structure of the human NLRP9 pyrin domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	125
Detector technology	PIXEL
Collection date	2019-12-08
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.000
Spacegroup name	C 1 2 1
Unit cell lengths	51.970, 33.685, 49.667
Unit cell angles	90.00, 102.40, 90.00

Refinement procedure

Resolution	28.080 - 2.080
Rwork	0.213
R-free	0.28250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ewi
RMSD bond length	0.015
RMSD bond angle	1.754
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.080	2.150
High resolution limit [Å]	2.080	2.100
Number of reflections	5035	306
<I/σ(I)>	22.28
Completeness [%]	98.9
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	20%(w/v) PEG MME 2,000 0.1M Tris pH 8.5 0.2M Trimethylamine n-oxide