7BSD

Complex structure of 1G5.3 Fab bound to ZIKV NS1c

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-03-22
Detector	ADSC QUANTUM 315
Wavelength(s)	1.03907
Spacegroup name	P 21 21 21
Unit cell lengths	108.889, 111.141, 158.587
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.500 - 2.530
R-factor	0.2217
Rwork	0.221
R-free	0.23880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5iy3
RMSD bond length	0.003
RMSD bond angle	0.707
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.154	1.621
Number of reflections	63619	62455
<I/σ(I)>	17.5	1.67
Completeness [%]	100.0	100
Redundancy	13.2	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	14% v/vPoly(acrylic acid-co-maleic acid) solution