7BSC

Complex structure of 1G5.3 Fab bound to DENV2 NS1c

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-03-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97774
Spacegroup name	P 21 2 21
Unit cell lengths	41.861, 80.980, 264.754
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.320 - 2.310
R-factor	0.207
Rwork	0.205
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o6b
RMSD bond length	0.004
RMSD bond angle	0.683
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	39747	3632
<I/σ(I)>	14.7
Completeness [%]	97.5
Redundancy	12.7
CC(1/2)	0.990	0.992

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.1 M DL-Malic acid pH 7.0, 12% w/v Polyethylene glycol 3350