7BRT

Crystal structure of Sec62 LIR fused to GABARAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2017-11-13
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	42.887, 42.889, 144.012
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.105 - 1.999
R-factor	0.1663
Rwork	0.166
R-free	0.18360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gnu
RMSD bond length	0.007
RMSD bond angle	0.906
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (dev_2450)
Refinement software	PHENIX (dev_2450)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.105	2.071
High resolution limit [Å]	1.999	1.999
Rmerge	0.164	0.920
Rmeas	0.178	0.178
Rpim	0.069	0.069
Number of reflections	18735	1782
<I/σ(I)>	9.27	2.02
Completeness [%]	99.7	98.34
Redundancy	6.5	5.8
CC(1/2)	0.996	0.759

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	1.5 M ammonium sulfate, 0.1 M Tris pH 8.5