7BRQ

Crystal structure of human FAM134B LIR fused to human GABARAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	95
Detector technology	PIXEL
Collection date	2017-10-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 63
Unit cell lengths	81.892, 81.892, 33.324
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.946 - 1.404
R-factor	0.1922
Rwork	0.192
R-free	0.20610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gnu
RMSD bond length	0.006
RMSD bond angle	0.790
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (dev_2450)
Refinement software	PHENIX (dev_2450)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.950	1.454
High resolution limit [Å]	1.404	1.404
Rmerge	0.074	0.631
Rmeas	0.078	0.683
Rpim	0.025	0.254
Number of reflections	24609	2058
<I/σ(I)>	18.8	2.68
Completeness [%]	97.7	82.68
Redundancy	9.7	6.5
CC(1/2)	0.997	0.755

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	1.91 M ammonium sulfate, 0.1 M sodium citrate pH 5.5, 0.18 M potassium sodium tartrate