7BR9

Crystal structure of mus musculus IRG1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	125
Detector technology	PIXEL
Collection date	2019-12-08
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	191.493, 55.610, 90.956
Unit cell angles	90.00, 107.67, 90.00

Refinement procedure

Resolution	29.450 - 3.300
R-factor	0.2208
Rwork	0.217
R-free	0.25680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6r6t
RMSD bond length	0.004
RMSD bond angle	0.807
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.450	3.360
High resolution limit [Å]	3.290	3.300
Number of reflections	13873	678
<I/σ(I)>	5.71
Completeness [%]	98.8
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.17M Lithium sulfate monohydrate 0.085M Tris-HCl pH 8.5 25.5% (w/v) PEG 4000 15% glycerol