7BPU

Structural and mechanistic insights into the biosynthesis of Digeranylgeranylglyceryl phosphate synthase in membranes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-10
Detector	DECTRIS PILATUS3 R 1M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 2
Unit cell lengths	70.360, 134.060, 79.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.530 - 3.320
R-factor	0.247
Rwork	0.246
R-free	0.27700
Structure solution method	SAD
Data reduction software	HKL-2000 (v1.0)
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX (1.11.1_2575:???)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.530	3.760
High resolution limit [Å]	3.320	3.520
Rmerge	0.200	0.467
Rmeas	0.218	0.507
Number of reflections	21570	3248
<I/σ(I)>	10.41	4.91
Completeness [%]	99.7	100
Redundancy	6.732	6.803
CC(1/2)	0.997	0.977

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293.15	200 mM NaCl, 20% (w/v) PEG500, and 200 mM Tris-HCl (pH 8.0).