7BPG
Structure of serinol nucleic acid - RNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2019-10-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.919744 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.363, 46.918, 47.540 |
| Unit cell angles | 90.00, 114.54, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.234 |
| Rwork | 0.232 |
| R-free | 0.27100 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.568 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.920 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.052 | 0.970 |
| Rmeas | 0.062 | 1.144 |
| Rpim | 0.032 | 0.600 |
| Number of reflections | 17920 | 917 |
| <I/σ(I)> | 14.3 | 2 |
| Completeness [%] | 99.8 | 98.5 |
| Redundancy | 3.5 | |
| CC(1/2) | 0.998 | 0.734 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 25% PEG 400, 0.1M HEPES (pH6.5), 75mM Calcium chloride |
