7BPF
Structure of L-threoninol nucleic acid - RNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2019-05-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.90000 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 52.996, 33.344, 54.055 |
| Unit cell angles | 90.00, 115.64, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.750 |
| R-factor | 0.27 |
| Rwork | 0.269 |
| R-free | 0.28800 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.655 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.500 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.060 | 0.307 |
| Rmeas | 0.079 | 0.397 |
| Rpim | 0.051 | 0.250 |
| Number of reflections | 8609 | 2064 |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 98.7 | 99.1 |
| Redundancy | 4.3 | |
| CC(1/2) | 0.989 | 0.944 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 28% PEG 400, 0.1M HEPES (pH 7.5), 0.2M Calcium chloride |
