7BPD
Crystal structural analysis of Swine dUTPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-10-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97835 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.175, 54.535, 52.107 |
| Unit cell angles | 90.00, 116.68, 90.00 |
Refinement procedure
| Resolution | 46.620 - 2.040 |
| R-factor | 0.21 |
| Rwork | 0.207 |
| R-free | 0.25750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hqu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.587 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.900 | 2.080 |
| High resolution limit [Å] | 2.030 | 2.040 |
| Rmerge | 0.058 | 0.297 |
| Rmeas | 0.074 | 0.968 |
| Rpim | 0.029 | 0.378 |
| Number of reflections | 35796 | 1301 |
| <I/σ(I)> | 23.67 | 2 |
| Completeness [%] | 83.0 | |
| Redundancy | 5.7 | |
| CC(1/2) | 0.992 | 0.972 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 200mM NaI, 20% (w/v) PEG 3350 |
