7BP2

Structural mechanism directing nucleosome reorganization by NAP1-RELATED PROTEIN 1 (NRP1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97853
Spacegroup name	P 31
Unit cell lengths	65.712, 65.712, 96.687
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.450 - 1.580
R-factor	0.1564
Rwork	0.155
R-free	0.18630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1aoi
RMSD bond length	0.018
RMSD bond angle	1.295
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.640
High resolution limit [Å]	1.580	3.400	1.580
Rmerge	0.049	0.037	0.299
Rmeas	0.052	0.039	0.321
Rpim	0.016	0.012	0.116
Number of reflections	64079	6436	6348
<I/σ(I)>	12.3
Completeness [%]	99.9	100	99.9
Redundancy	8.7	10.2	7.1
CC(1/2)		0.999	0.932

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.2M Sodium sulphate 0.1M Bis Tris propane pH 7.5 20% w/v PEG 3350