7BOV

The Structure of Bacillus subtilis glycosyltransferase,Bs-YjiC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97891
Spacegroup name	I 2 2 2
Unit cell lengths	51.882, 118.433, 184.098
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.620 - 2.293
R-factor	0.2368
Rwork	0.234
R-free	0.26580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2iya
RMSD bond length	0.017
RMSD bond angle	1.685
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.620	2.375
High resolution limit [Å]	2.293	2.293
Rmeas		0.804
Number of reflections	47727	2446
<I/σ(I)>	15.21
Completeness [%]	97.6
Redundancy	9.3
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291.15	2mM phloretin;5mM GDP; 30% PEG 3350;150mM NaCl;100mM Bis-Tris