7BOP

Crystal Structure of Core-mannan synthase A (CmsA/Ktr4) from Aspergillus fumigatus, Mn/GDP-form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9
Spacegroup name	C 2 2 21
Unit cell lengths	154.504, 273.006, 186.279
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.820 - 1.900
R-factor	0.16132
Rwork	0.160
R-free	0.18120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a07
RMSD bond length	0.008
RMSD bond angle	1.304
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.820	1.968
High resolution limit [Å]	1.900	1.900
Rmerge	0.066	0.734
Rmeas	0.072	0.792
Number of reflections	306207	30240
<I/σ(I)>	15.36	1.91
Completeness [%]	99.9
Redundancy	6.9
CC(1/2)	0.999	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	sodium citrate