7BOO

Crystal Structure of Core-mannan synthase A (CmsA/Ktr4) from Aspergillus fumigatus, apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9
Spacegroup name	C 2 2 21
Unit cell lengths	154.719, 274.296, 185.974
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.990 - 1.950
R-factor	0.1644
Rwork	0.163
R-free	0.18594
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a07
RMSD bond length	0.008
RMSD bond angle	1.290
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.990	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.062	0.555
Rmeas	0.067	0.600
Number of reflections	284394	27979
<I/σ(I)>	19.22	3.38
Completeness [%]	99.8
Redundancy	7
CC(1/2)	0.999	0.897

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	sodium citrate