7BNW

Llama nanobody nb18 raised against GldL from Flavobacterium jonhsoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978
Spacegroup name	P 65
Unit cell lengths	95.857, 95.857, 118.895
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.450 - 2.590
R-factor	0.1951
Rwork	0.193
R-free	0.24270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t2j
RMSD bond length	0.013
RMSD bond angle	1.778
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.450	2.750
High resolution limit [Å]	2.590	2.590
Number of reflections	19199	3027
<I/σ(I)>	9
Completeness [%]	99.8
Redundancy	6.5
CC(1/2)	0.994	0.460

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	100mM sodium acetate pH 4.5, 200mM calcium acetate, 30% PEG 400