7BNR

Crystal structure of a ParB Q52A mutant from Myxococcus xanthus bound to CTPyS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97626
Spacegroup name	P 21 21 21
Unit cell lengths	45.750, 80.870, 135.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.350 - 1.700
R-factor	0.1837
Rwork	0.182
R-free	0.21070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bnk
RMSD bond length	0.007
RMSD bond angle	1.253
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.390	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.060
Number of reflections	56287	7929
<I/σ(I)>	19.67	0.74
Completeness [%]	99.8
Redundancy	12.8
CC(1/2)	1.000	0.457

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris pH 8.5, 20% PEG 1000