7BMY
p62PH in cesium chloride (0.7 M CsCl in crystallization condition and cryo protectant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.77114 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 34.426, 106.791, 75.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.670 - 1.800 |
| R-factor | 0.1847 |
| Rwork | 0.183 |
| R-free | 0.21450 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.108 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.670 | 43.670 | 1.840 |
| High resolution limit [Å] | 1.800 | 9.000 | 1.800 |
| Rmerge | 0.150 | 0.105 | 1.359 |
| Rmeas | 0.152 | 0.107 | 1.392 |
| Rpim | 0.026 | 0.018 | 0.293 |
| Total number of observations | 4358 | 10544 | |
| Number of reflections | 12670 | 137 | 487 |
| <I/σ(I)> | 22 | 50.8 | 2.7 |
| Completeness [%] | 94.9 | 99.5 | 65.1 |
| Redundancy | 33 | 31.8 | 21.7 |
| CC(1/2) | 0.999 | 0.999 | 0.851 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.7 M cesium chloride, 18 % (w/v) PEG 4000 |
