7BMX

p62PH in cesium chloride (0.25 M CsCl in protein buffer and 0.75 M CsCl in cryo protectant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.7712
Spacegroup name	C 2 2 21
Unit cell lengths	34.274, 106.783, 75.319
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.560 - 1.900
R-factor	0.1858
Rwork	0.184
R-free	0.21640
Structure solution method	SAD
RMSD bond length	0.019
RMSD bond angle	1.940
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXDE
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.560	43.560	1.940
High resolution limit [Å]	1.900	9.110	1.900
Rmerge	0.071	0.067	0.266
Rmeas	0.074	0.070	0.278
Rpim	0.021	0.021	0.080
Total number of observations		1421	7410
Number of reflections	10885	129	658
<I/σ(I)>	25.5	45.2	9.2
Completeness [%]	96.3	99.5	92.5
Redundancy	11.5	11	11.3
CC(1/2)	0.999	0.997	0.983

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.9 M potassium chloride, 17 % (w/v) PEG 4000