7BM6

Structure-function analysis of a new PL17 oligoalginate lyase from the marine bacterium Zobellia galactanivorans DsijT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-25
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.98
Spacegroup name	P 65
Unit cell lengths	164.132, 164.132, 168.370
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.000 - 2.160
R-factor	0.1547
Rwork	0.153
R-free	0.18220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bjt
RMSD bond length	0.016
RMSD bond angle	1.688
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.210
High resolution limit [Å]	2.160	2.160
Rmerge		1.570
Rpim	0.054	0.356
Number of reflections	137171	7343
<I/σ(I)>	16.3
Completeness [%]	99.6	99.2
Redundancy	21.16	18
CC(1/2)	0.999	0.641

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	296	protein solution at 13.4 mg.ml-1, supplemented with 1.6 mM of oligo-mannuronate of DP3, was mixed with 0.2 micro-l of crystallization solution that contained 2.4 M sodium malonate (dibasic monohydrate) and equilibrated against a reservoir containing 100 micro-l.