7BIP

Crystal structure of monooxygenase RslO1 from Streptomyces bottropensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	50.912, 81.962, 160.624
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.530 - 1.600
R-factor	0.1659
Rwork	0.164
R-free	0.19390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2i7g
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MrBUMP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.530	1.630
High resolution limit [Å]	1.600	1.600
Number of reflections	89593	4319
<I/σ(I)>	13.3
Completeness [%]	100.0
Redundancy	25.8
CC(1/2)	0.999	0.542

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M calcium chloride, 0.1 M HEPES pH 7.0 and 33% polyethylene glycol