7BIH
Crystal structure of RecJ-Cdc45 from Methanothermobacter Thermoautotrophicus in the closed state.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.149, 101.629, 91.078 |
Unit cell angles | 90.00, 96.08, 90.00 |
Refinement procedure
Resolution | 41.300 - 3.000 |
R-factor | 0.2143 |
Rwork | 0.210 |
R-free | 0.28260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6tvv |
RMSD bond length | 0.011 |
RMSD bond angle | 1.492 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.500 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 19632 | 2851 |
<I/σ(I)> | 4.5 | |
Completeness [%] | 99.8 | |
Redundancy | 8.7 | |
CC(1/2) | 0.990 | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Tris/HCl pH 8.5, 27% w/v PEG 4000, 0.2M MgCl2*6H20, 1mM MnCl2 |