7BGS

Archeal holliday junction resolvase from Thermus thermophilus phage 15-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	97.580, 103.710, 84.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.150 - 2.500
R-factor	0.2372
Rwork	0.236
R-free	0.25810
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	0.940
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	BUSTER (2.11.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.200	2.600
High resolution limit [Å]	2.500	2.500
Number of reflections	253474	17436
<I/σ(I)>	18.2
Completeness [%]	99.9
Redundancy	16.7
CC(1/2)	0.999	0.631

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.8 M ammonium sulphate and 0.1 M sodium acetate pH 4.2