7BGO

The crystal structure of gene product PA4063 from Pseudomonas aeruginosa in complex with Zn (space group P65)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.27110
Spacegroup name	P 65
Unit cell lengths	122.005, 122.005, 102.263
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.940 - 3.301
Rwork	0.177
R-free	0.21140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7aly
RMSD bond length	0.010
RMSD bond angle	1.785
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.940	46.940	3.570
High resolution limit [Å]	3.300	8.730	3.300
Rmerge	0.135	0.030	2.461
Rmeas	0.143	0.032	2.608
Rpim	0.047	0.011	0.860
Number of reflections	12599	733	2650
<I/σ(I)>	18.6
Completeness [%]	96.1
Redundancy	17.7	15.8	17.9
CC(1/2)	0.999	1.000	0.677

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	AS 3M, MPD 1%