7BG1
Structure of anti-FLAG M2 Fab domain remodeled based on proteomic sequencing
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-17 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 87.360, 133.760, 41.480 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.520 - 1.860 |
R-factor | 0.2192 |
Rwork | 0.217 |
R-free | 0.25520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g60 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.727 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.033 | 0.249 |
Number of reflections | 46087 | 2659 |
<I/σ(I)> | 19 | |
Completeness [%] | 92.3 | 58.9 |
Redundancy | 4.2 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 15% PEG 4000, 0.1M ammonium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |