7BFL

X-ray structure of SS-RNase-2 des116-120

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2020-07-06
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	110.070, 93.070, 66.780
Unit cell angles	90.00, 117.19, 90.00

Refinement procedure

Resolution	67.460 - 2.880
R-factor	0.2613
Rwork	0.259
R-free	0.30760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bfk
RMSD bond length	0.003
RMSD bond angle	1.143
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.000	3.060
High resolution limit [Å]	2.880	2.880
Number of reflections	13043	1832
<I/σ(I)>	3.4	1.2
Completeness [%]	96.2
Redundancy	3.4
CC(1/2)	0.889	0.411

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	25% w/v PEG 3350, 0.2 M sodium chloride and 0.1 M Bis-Tris pH 6.5