7BEW
Glyceraldehyde 3-phosphate dehydrogenase from Campylobacter jejeuni - NAD(P) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 90.810, 90.810, 224.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.797 - 2.250 |
| Rwork | 0.163 |
| R-free | 0.21180 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.674 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.210 | 2.350 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.150 | 0.730 |
| Number of reflections | 22791 | 1658 |
| <I/σ(I)> | 10.6 | 2.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 295 | PEG 4000 (20%), 0.3 M Na/K tartrate, 01.M Na Acetate |
