7BEC
Unusual structural features in the adduct of dirhodium tetraacetate with lysozyme (5)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.700, 78.700, 37.245 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.190 - 1.740 |
| Rwork | 0.185 |
| R-free | 0.22840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 193l |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.553 |
| Data reduction software | MOSFLM |
| Data scaling software | MOSFLM |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.190 | 1.830 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.070 | 3.120 |
| Rpim | 0.020 | 0.848 |
| Number of reflections | 11349 | 567 |
| <I/σ(I)> | 24.7 | 1.1 |
| Completeness [%] | 91.1 | 36 |
| Redundancy | 25 | 27.6 |
| CC(1/2) | 1.000 | 0.474 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.1 M sodium acetate, 20% ethylene glicol, 0.1 M sodium nitrate |
