7BE2

X-ray structure of Hen Egg White Lysozyme with dirhodium tetraacetate (6)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.5406
Spacegroup name	P 43 21 2
Unit cell lengths	78.930, 78.930, 37.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.810 - 1.650
Rwork	0.182
R-free	0.23410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	193l
RMSD bond length	0.008
RMSD bond angle	1.546
Data reduction software	MOSFLM
Data scaling software	MOSFLM
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.812	1.800
High resolution limit [Å]	1.650	1.650
Rmerge	0.120	3.230
Rpim	0.025	0.680
Number of reflections	11800	591
<I/σ(I)>	22.5	1.3
Completeness [%]	94.0	52.6
Redundancy	24.5	22.4
CC(1/2)	1.000	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.01 M HEPES, 2.00 M sodium formate