7BDU

Crystal structure of a Hsp47-collagen peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-24
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	91.821, 129.800, 173.372
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.910 - 2.490
R-factor	0.2201
Rwork	0.217
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4au2
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.910	2.590
High resolution limit [Å]	2.490	2.490
Rmerge	0.120	0.800
Rmeas	0.125
Number of reflections	68330	3406
<I/σ(I)>	10.12
Completeness [%]	99.0
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M HEPES, 18-26% PEG 3350, 1-6% Tacsimate pH 7.0