7BDQ
MAPK14 bound with SR300
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.027, 73.041, 74.347 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.900 - 2.750 |
| R-factor | 0.2178 |
| Rwork | 0.215 |
| R-free | 0.26160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lar |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.399 |
| Data reduction software | Aimless (0.7.4) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.950 | 48.950 | 2.900 |
| High resolution limit [Å] | 2.750 | 8.700 | 2.750 |
| Rmerge | 0.217 | 0.091 | 1.544 |
| Rmeas | 0.244 | 0.103 | 1.735 |
| Rpim | 0.110 | 0.047 | 0.774 |
| Total number of observations | 1414 | 6284 | |
| Number of reflections | 9622 | 361 | 1376 |
| <I/σ(I)> | 5.9 | 8.8 | 2.7 |
| Completeness [%] | 99.6 | 99.6 | 99.7 |
| Redundancy | 4.4 | 3.9 | 4.6 |
| CC(1/2) | 0.977 | 0.987 | 0.446 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 14% HMW PgSmear, 0.1M MES |
