7BCY

X-ray structure of WDR5delta24 bound to the Kaposi's sarcoma herpesvirus LANA win motif peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9762
Spacegroup name	P 1
Unit cell lengths	46.313, 53.171, 65.124
Unit cell angles	72.58, 89.75, 73.16

Refinement procedure

Resolution	39.000 - 1.500
R-factor	0.1949
Rwork	0.193
R-free	0.22680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ery
RMSD bond length	0.011
RMSD bond angle	1.156
Data reduction software	DIALS (1.10.0-gf2f43cdc6)
Data scaling software	Aimless (0.7.1)
Phasing software	MOLREP (11.6.04)
Refinement software	PHENIX (1.19rc7_4070)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.936	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.068	0.380
Rmeas	0.082	0.461
Rpim	0.046	0.258
Number of reflections	78054	3941
<I/σ(I)>	7.8	2.1
Completeness [%]	86.3	87.4
Redundancy	3.1	3.1
CC(1/2)	0.993	0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	294	100 mM Bis-Tris pH 5.5, 50 mM (NH4)2SO4, 25% PEG3350