7BCM
The DDR1 Kinase Domain Bound To SR302
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-28 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.223, 120.402, 62.095 |
| Unit cell angles | 90.00, 111.35, 90.00 |
Refinement procedure
| Resolution | 41.710 - 2.300 |
| R-factor | 0.2462 |
| Rwork | 0.243 |
| R-free | 0.30800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bkj |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.511 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (7.0.053) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.710 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.082 | 0.659 |
| Number of reflections | 33763 | 3333 |
| <I/σ(I)> | 8.3 | 2.2 |
| Completeness [%] | 98.9 | |
| Redundancy | 3.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 20% PEG10,000 0.1 M HEPES pH 7.5 |
