7BCA

Crystal structure of the HTH DNA binding protein ArdK from R388 plasmid bound to a direct-repeat DNA element

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	105
Detector technology	CCD
Collection date	2009-05-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9790
Spacegroup name	C 1 2 1
Unit cell lengths	88.120, 39.060, 120.040
Unit cell angles	90.00, 101.65, 90.00

Refinement procedure

Resolution	58.780 - 2.800
R-factor	0.2345
Rwork	0.232
R-free	0.29260
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.720
Data reduction software	MOSFLM (7.2.2)
Data scaling software	SCALA (CCP4 7.0.078)
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.780	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.051	0.335
Rmeas	0.071	0.462
Number of reflections	10025	1437
<I/σ(I)>	10.4	2.7
Completeness [%]	98.9
Redundancy	3.4
CC(1/2)	0.997	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	20% PEG 4,000, 10% 2-propanol, 0.1 M Tris-HCl [pH 7.5]