7BBL
Structure of human Gemin6/Gemin7/Gemin8 trimeric complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2016-11-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 59.880, 80.597, 82.666 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.494 - 1.521 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.25060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y96 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.1_3469) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.494 | 1.576 |
| High resolution limit [Å] | 1.521 | 1.521 |
| Rmerge | 0.067 | 0.793 |
| Rmeas | 0.076 | 0.960 |
| Rpim | 0.035 | 0.523 |
| Number of reflections | 58263 | 4340 |
| <I/σ(I)> | 10.83 | 1.03 |
| Completeness [%] | 93.5 | 70.86 |
| Redundancy | 3.8 | 2.3 |
| CC(1/2) | 0.999 | 0.197 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 100 mM 2-(N-morpholino) ethanesulfonic acid, 200 mM NaCl, 30 % Jeffamine ED2003 |
