7BB3

Structure of S. pombe YG-box oligomer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	77
Detector technology	PIXEL
Collection date	2016-11-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976251
Spacegroup name	C 2 2 21
Unit cell lengths	27.190, 83.710, 160.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.493 - 2.158
R-factor	0.2586
Rwork	0.257
R-free	0.29190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rg5
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15.1_3469)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.493	2.235
High resolution limit [Å]	2.158	2.158
Rmerge	0.105	0.824
Rmeas	0.114	0.891
Rpim	0.045	0.334
Number of reflections	10282	1010
<I/σ(I)>	10.85	2.25
Completeness [%]	99.4	99.7
Redundancy	6.6
CC(1/2)	0.998	0.708

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	289.15	65 % 2-methylpentanediol, 80 mM KCl, 40 mM HEPES